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I am writing the listserve to request information about personal experience and opinion regarding the Instrumedics CryoJane tape system for preparing cryosections for confocal microscopy analysis.
Specifically,
1. does it do a better job of retaining antigenicity in tissues that contain bone since you do not have to use reagents to decalcify before cutting them?
2. Does it work as nicely as it was shown to in the demonstration at the Neuroscience meeting in San Diego this year?
3. Is it worth the expenditure of money? Apparently it requires a motorized sectioning cryostat to be really effective in cutting through tissue that contains bone or through whole embryos.
4. Other relevant information positive or negative regarding its use for immunofluorescent analyses.
Thanks,
Mary Ann Asson-Batres
Mary Ann Asson-Batres, PhD
Associate Professor
Department of Biological Sciences
Tennessee State University
PO Box 1116 (postal mail)
Room 313 Harned Hall (courier)
3500 John A Merritt Blvd
Nashville, TN 37209
Office 615-963-5779
FAX 615-963-2142
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