Dear Page
I worked for Leica for awhile and have a
lot of experience on the SP2, I know there is some variability with the FRET
calculations. With that said, there is probably one of the best FRET guys
I have ever worked with at Leica , his name is Scott Young and he can be
reached in there Exton Pa office (provided he is still with the company but I believe
he is) – he is really good with this, worth a phone call - I think
you can reach him at the leica one call number -
Hope this helps – I think given my
experience with working for Leica in the past they will go out of their way to
help you
Regards
Brian
Dr. Brian T. Bennett
Chief Scientist - Microscopy
518-523-7025 X202
From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of D. Page Baluch
Sent: Monday, November 26, 2007
2:37 PM
To: [hidden email]
Subject: FRET in publications
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I have a question in
regards to publishing FRET data. Our University has a Leica SP2 confocal
microscope that calculates the FRET percentage values and I have used this data
in publications where experimental and control values were measured but
recently I submitted a publication and was asked to provide more information regarding
this data. A reviewer asked that FRET traces be shown and more explanation as
to the degree of interaction. I was curious on how others reported this data
and what additional information you also present with your percent FRET values.
Thanks for your assistance,
Page
--
Page Baluch, Ph.D.
Arizona State University/SoLS
480-965-7011
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