Re: How to make a Leica Series from Single Leica Images?

Posted by Chris Tully on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-make-a-Leica-Series-from-Single-Leica-Images-tp591100p591105.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal You can also try Image-Pro Plus.  The latest version (6.2) has a new feature in the File Open dialog: a check box "Open as Sequence" that will open any selected set of images as a sequence that can be played as a time series in the base product or render as a 3D volume using the 3D Constructor plug-in for Image-Pro Plus.  Image-Pro Plus will also automatically import the spatial and and temporal data from each image as standard TIFF properties that will be retained if the sequence is then resaved as a TIFF sequence.

Chris Tully
Applications Engineer
Vashaw Scientific, Inc.
www.vashaw.com
[hidden email] (work)
[hidden email] (personal)

On Dec 4, 2007 12:25 PM, Glen MacDonald <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You could try ImageJ, use File/Import/Image Sequence... to import a
sequentially numbered series into a stack.  Use the Image/Properties
window to set spatial and temporal dimensions.
More explanation is in the ImageJ documentations.
rsb.info.nih.gov/ij
Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

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On Dec 4, 2007, at 1:16 AM, Tino Jäger wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anyone have an idea, how to make a Leica Series out of single
> Leica-Tif-Images (Leica SP2 & LCS)?
> I  know about the merge-option in the Leica Confocal Software, but
> there its only possible to add one single image.
> A painful work if you need to merge hundrets of images...
>
> Does anyone have an idea how to do this job?
> Is it possible to merge more then one tif  the same time in the LCS?
> Is it possible so save a merged series in ImageJ as a Leica Series?
> Does anyone have a VisualBasics-Macro for that, and is willing to
> share?
> Does anyone have another idea?
>
> Thanks for your help,
> Tino
>
>
>
> --
> Dr. Tino Jäger
> Department of Neurophysiology Center for Brain Research
> Medical University of Vienna Spitalgasse 4, A-1090 Vienna
>
> Tel:  [phone]
> Secr: [phone] FAX:  [phone]
>
> http://www.meduniwien.ac.at