Re: not a confocal question - features of a widefield

Posted by Nuno Moreno on
URL: http://confocal-microscopy-list.275.s1.nabble.com/not-a-confocal-question-features-of-a-widefield-tp591195p591198.html

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Autoexpose will bleach everything, right?

Regarding the adaptative focus that I mentioned before, there are
commercial system that with minimum light and before an acquisition
"measure" the cell position and adapt the focus. But this is an half
adaptation. It could be that it does not need to readjust the focus.

What I was counting with would be after the acquisition, if it is out of
focus, it make the adjustment base in some kind of sensitivity
parameter. This could be after 10 time points but it might be that it
would never need such adjustment.


About the intensity variations I'm not talking about post processing
adjustments. If it gets saturated there are no post processing that can
help you.

Regards,
NM





Shalin Mehta wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Nuno,
>
>  Wouldn't auto-exposure on cameras suffice for maintaining constant
> intensity?
>
> Apparently most of the commercial adaptive optics systems are geared
> towards astronomy. Perhaps you have known this already:
> http://cfao.ucolick.org/
> Interesting to note that James Webb space telescope will have hardware
> and intelligence for adaptive optics evolved from algorithms developed
> for correcting aberrations for hubble telescope.
>
> Regards,
> Shalin
>
>
> On Dec 7, 2007 10:43 PM, Nuno Moreno <[hidden email]
> <mailto:[hidden email]>> wrote:
>
>     Search the CONFOCAL archive at
>     http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>     Does anyone knows any commercial widefield SYSTEM that makes an
>     adaptative focus. And I mean adaptative (follows the cell.
>
>     The other feature is a commercial system that keeps intensities, i.e.,
>     if you have something with different protein expression levels over
>     time, the system will correct the exposure time so that at the end the
>     intensities are constant.
>
>     Many thanks,
>     --
>     Nuno Moreno
>     Cell Imaging Unit
>     Instituto Gulbenkian de Ciência
>     http://uic.igc.gulbekian.pt <http://uic.igc.gulbekian.pt>
>     http://www.igc.gulbekian.pt
>     phone [phone]
>     fax   [phone]
>
>
>
>
> --
> ~~~~~~~~~~~~~~~~~~~~~~~~~
> Shalin Mehta
> Graduate Student in Bioengineering, NUS
> mobile: [phone]
> blog: shalin.wordpress.com <http://shalin.wordpress.com>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~

--
Nuno Moreno
Cell Imaging Unit
Instituto Gulbenkian de Ciência
http://uic.igc.gulbekian.pt
http://www.igc.gulbekian.pt
phone [phone]
fax   [phone]