Re: not a confocal question - features of a widefield

Posted by Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/not-a-confocal-question-features-of-a-widefield-tp591195p591208.html

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On Dec 7, 2007, at 2:03 PM, Nuno Moreno wrote:

Eventually, she did what you

suggest, except that she was part of the feed back loop: she just kept looking at the images on the monitor as they were being acquired and manually refocusing the microscope. Couldn't find any software that would do that better than she did...

Yes but if your timelapse is more than 10 hours...

Even so, it should be no problem...  you can always find motivated grad students. Besides, a one year grad student stipend  costs about the same as a 1-year maintenance agreement for some of the imaging software packages we own...

Just (barely) kidding!

Julio.