http://confocal-microscopy-list.275.s1.nabble.com/not-a-confocal-question-features-of-a-widefield-tp591195p591215.html
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> Nikon has a Perfect Focus System I believe for their TE2000 series. It
> uses an LED (770nm) to focus on the coverslip and adjust Z prior to
> image acquisition.
>
http://nikonusa.com/fileuploads/pdfs/Instruments/TE2000_PFS.pdf>
> I haven't tried it but sounds similar to what you are looking for and
> could use their system as an example for building your own.
>
> -Lars
>
> ------------------------------------------------------------------------
> *From:* Confocal Microscopy List [mailto:
[hidden email]]
> *On Behalf Of *Zoltan Cseresnyes
> *Sent:* Friday, December 07, 2007 9:50 AM
> *To:*
[hidden email]
> *Subject:* Re: not a confocal question - features of a widefield
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> I've been thinking about building a system like that ever since one of
> our users started doing overnight recordings and his samples sometimes
> drift vertically and thus would need an autofocus system to correct for
> that. If you could tap into the Z-controller circuit of your system,
> you would be able to drive that with a signal that's proportional to the
> vertical drift. The drift could be scaled with e.g. calculating average
> intensity during a Z-stack from a certain area of the image that's
> fairly thin. Similar logic would work for the autoexposure as well.
> Want to try it?
>
> Zoltan
>
> On Dec 7, 2007 4:41 PM, Nuno Moreno <
[hidden email]
> <mailto:
[hidden email]>> wrote:
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Autoexpose will bleach everything, right?
>
> Regarding the adaptative focus that I mentioned before, there are
> commercial system that with minimum light and before an acquisition
> "measure" the cell position and adapt the focus. But this is an half
> adaptation. It could be that it does not need to readjust the focus.
>
> What I was counting with would be after the acquisition, if it is out of
> focus, it make the adjustment base in some kind of sensitivity
> parameter. This could be after 10 time points but it might be that it
> would never need such adjustment.
>
>
> About the intensity variations I'm not talking about post processing
> adjustments. If it gets saturated there are no post processing that can
> help you.
>
> Regards,
> NM
>
>
>
>
>
> Shalin Mehta wrote:
> > Search the CONFOCAL archive at
> >
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> <
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>Dear Nuno,
> >
> > Wouldn't auto-exposure on cameras suffice for maintaining constant
> > intensity?
> >
> > Apparently most of the commercial adaptive optics systems are geared
> > towards astronomy. Perhaps you have known this already:
> >
http://cfao.ucolick.org/> > Interesting to note that James Webb space telescope will have
> hardware
> > and intelligence for adaptive optics evolved from algorithms
> developed
> > for correcting aberrations for hubble telescope.
> >
> > Regards,
> > Shalin
> >
> >
> > On Dec 7, 2007 10:43 PM, Nuno Moreno <
[hidden email]
> <mailto:
[hidden email]>
> > <mailto:
[hidden email]
> <mailto:
[hidden email]>>> wrote:
> >
> > Search the CONFOCAL archive at
> >
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> >
> > Does anyone knows any commercial widefield SYSTEM that makes an
> > adaptative focus. And I mean adaptative (follows the cell.
> >
> > The other feature is a commercial system that keeps
> intensities, i.e.,
> > if you have something with different protein expression
> levels over
> > time, the system will correct the exposure time so that at
> the end the
> > intensities are constant.
> >
> > Many thanks,
> > --
> > Nuno Moreno
> > Cell Imaging Unit
> > Instituto Gulbenkian de Ciência
> >
http://uic.igc.gulbekian.pt <
http://uic.igc.gulbekian.pt/>
> <
http://uic.igc.gulbekian.pt <
http://uic.igc.gulbekian.pt/>>
> >
http://www.igc.gulbekian.pt <
http://www.igc.gulbekian.pt/>
> > phone +351 214464606
> > fax +351 214407970
> >
> >
> >
> >
> > --
> > ~~~~~~~~~~~~~~~~~~~~~~~~~
> > Shalin Mehta
> > Graduate Student in Bioengineering, NUS
> > mobile: +65-90694182
> > blog: shalin.wordpress.com
> <
http://shalin.wordpress.com/><
http://shalin.wordpress.com> <
http://shalin.wordpress.com/>>
> > ~~~~~~~~~~~~~~~~~~~~~~~~~~
>
> --
> Nuno Moreno
> Cell Imaging Unit
> Instituto Gulbenkian de Ciência
>
http://uic.igc.gulbekian.pt <
http://uic.igc.gulbekian.pt/>
>
http://www.igc.gulbekian.pt <
http://www.igc.gulbekian.pt/>
> phone +351 214464606
> fax +351 214407970
>
>
>
>
> --
> --
> Zoltan Cseresnyes
> Facility manager, Imaging Suite
> Dept. of Zoology University of Cambridge
> Downing Street, Cambridge
> CB2 3EJ UK
>
> Tel.: (++44) (0)1223 769282
> Fax.: (++44) (0)1223 336676