http://confocal-microscopy-list.275.s1.nabble.com/not-a-confocal-question-features-of-a-widefield-tp591195p591216.html
Been following this string . If this is such a big problem, everyone should be on the TiPFS system. New system is absolutely perfect.
BTW, how come you'r not down in the islands? I need a place to go to soon. Visiting friends in NC this weekend after the ASCB meeting, saw Barbara .
>Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
>Yes. In fact I just did it and I was wondering if there was some kind
>of commercial solution to compare.
>
>Even Deltavison what they do is having a switchable auto-focus that
>exposes with low light before taking the "real" picture, which is not
>adaptative. It might work better than mine because thinks are better
>integrated but it is not more elegant :)
>
>The adaptative exposure its so stupidly simple that I don't know why it
>is not integrated the most of the well know software. Not IPP,
>metamorph , Leica, Zeiss or Deltavision...as far has I know.
>
>Maybe I have to sell it ;).
>
>Regards,
>NM
>
>
>
>Zoltan Cseresnyes wrote:
>>Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>I've been thinking about building a system like that ever since one of
>>our users started doing overnight recordings and his samples sometimes
>>drift vertically and thus would need an autofocus system to correct
>>for that. If you could tap into the Z-controller circuit of your
>>system, you would be able to drive that with a signal that's
>>proportional to the vertical drift. The drift could be scaled with
>>e.g.
>>calculating average intensity during a Z-stack from a certain area of
>>the image that's fairly thin. Similar logic would work for the
>>autoexposure as well.
>>Want to try it? Zoltan
>>On Dec 7, 2007 4:41 PM, Nuno Moreno
>><
[hidden email] <mailto:
[hidden email]>> wrote:
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>> Autoexpose will bleach everything, right?
>> Regarding the adaptative focus that I mentioned before, there are
>> commercial system that with minimum light and before an acquisition
>> "measure" the cell position and adapt the focus. But this is an half
>> adaptation. It could be that it does not need to readjust the focus.
>> What I was counting with would be after the acquisition, if it is out of
>> focus, it make the adjustment base in some kind of sensitivity
>> parameter. This could be after 10 time points but it might be that it
>> would never need such adjustment.
>>
>> About the intensity variations I'm not talking about post processing
>> adjustments. If it gets saturated there are no post processing that can
>> help you.
>> Regards,
>> NM
>>
>>
>> Shalin Mehta wrote:
>> > Search the CONFOCAL archive at
>> >
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>> <
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> Dear Nuno,
>> >
>> > Wouldn't auto-exposure on cameras suffice for maintaining constant
>> > intensity?
>> >
>> > Apparently most of the commercial adaptive optics systems are geared
>> > towards astronomy. Perhaps you have known this already:
>> >
http://cfao.ucolick.org/>> > Interesting to note that James Webb space telescope will have
>> hardware
>> > and intelligence for adaptive optics evolved from algorithms
>> developed
>> > for correcting aberrations for hubble telescope.
>> >
>> > Regards,
>> > Shalin
>> >
>> >
>> > On Dec 7, 2007 10:43 PM, Nuno Moreno <
[hidden email]
>> <mailto:
[hidden email]>
>> >
>> <mailto:
[hidden email]>
>> <mailto:
[hidden email]>>> wrote:
>> >
>> > Search the CONFOCAL archive at
>> >
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>> >
>> > Does anyone knows any commercial widefield SYSTEM that makes an
>> > adaptative focus. And I mean adaptative (follows the cell.
>> >
>> > The other feature is a commercial system that keeps
>> intensities, i.e.,
>> > if you have something with different protein expression
>> levels over
>> > time, the system will correct the exposure time so that at
>> the end the
>> > intensities are constant.
>> >
>> > Many thanks,
>> > --
>> > Nuno Moreno
>> > Cell Imaging Unit
>> > Instituto Gulbenkian de Ciência
>> >
http://uic.igc.gulbekian.pt <
http://uic.igc.gulbekian.pt/>
>> <
http://uic.igc.gulbekian.pt <
http://uic.igc.gulbekian.pt/>>
>> >
http://www.igc.gulbekian.pt <
http://www.igc.gulbekian.pt/>
>> > phone +351 214464606
>> > fax +351 214407970
>> >
>> >
>> >
>> >
>> > --
>> > ~~~~~~~~~~~~~~~~~~~~~~~~~
>> > Shalin Mehta
>> > Graduate Student in Bioengineering, NUS
>> > mobile: +65-90694182
>> > blog: shalin.wordpress.com <
http://shalin.wordpress.com/>
>> <
http://shalin.wordpress.com <
http://shalin.wordpress.com/>>
>> > ~~~~~~~~~~~~~~~~~~~~~~~~~~
>> --
>> Nuno Moreno
>> Cell Imaging Unit
>> Instituto Gulbenkian de Ciência
>>
http://uic.igc.gulbekian.pt <
http://uic.igc.gulbekian.pt/>
>>
http://www.igc.gulbekian.pt <
http://www.igc.gulbekian.pt/>
>> phone +351 214464606
>> fax +351 214407970
>>
>>
>>--
>>--
>>Zoltan Cseresnyes
>>Facility manager, Imaging Suite
>>Dept. of Zoology University of Cambridge Downing Street, Cambridge
>>CB2 3EJ UK
>>Tel.: (++44) (0)1223 769282
>>Fax.: (++44) (0)1223 336676
>
>--
>Nuno Moreno
>Cell Imaging Unit
>Instituto Gulbenkian de Ciência
>
http://uic.igc.gulbekian.pt>
http://www.igc.gulbekian.pt>phone +351 214464606
>fax +351 214407970
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