Re: not a confocal question - features of a widefield

Posted by Nuno Moreno on
URL: http://confocal-microscopy-list.275.s1.nabble.com/not-a-confocal-question-features-of-a-widefield-tp591195p591218.html

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Yes, but again autofocus is a concept that I never liked. This means
exposing your cells to more light...and it might be that cells are just
at the same place!

Regards,
NM

Engstrom, Lars wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I just re-read your message and realize you are looking for cell tracking ability. I know MediaCybernetics acquisition software QED has a cell tracking function. I never used it and don't think it included an autofocus but I really liked their user interface and many of their features.
> http://www.mediacy.com/index.aspx?page=InVivo
> No commercial interest.
>
> -Lars  
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Nuno Moreno
> Sent: Friday, December 07, 2007 6:43 AM
> To: [hidden email]
> Subject: not a confocal question - features of a widefield
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anyone knows any commercial widefield SYSTEM that makes an
> adaptative focus. And I mean adaptative (follows the cell.
>
> The other feature is a commercial system that keeps intensities, i.e.,
> if you have something with different protein expression levels over
> time, the system will correct the exposure time so that at the end the
> intensities are constant.
>
> Many thanks,

--
Nuno Moreno
Cell Imaging Unit
Instituto Gulbenkian de Ciência
http://uic.igc.gulbekian.pt
http://www.igc.gulbekian.pt
phone +351 214464606
fax   +351 214407970