Hi Dave,
we recently tested the
Visitech Infinity and compared it with “conventional” spinning disc
microscopes.
Depending on the specimen
it definitively performs as well/as bad as a spinning disk microscope. We
obtained really good results with more or less flat cells (HeLa cells). The
results obtained with zebra fish as a sample were a little bit less convincing.
We made the tests with an EM-CCD camera (Cascade II, roper Scientific, 16 µm
pixels). Drawback of this approach is that one can only use high magnification
objectives to do Nyquist sampling. Thus to make uses of the adjustable pinholes
of the system one would have to use a different (less sensitive) camera.
If you have further
questions you can contact me also off-list.
Cheers Arne
(no commercial interests)
____________________________________________________
Dr. Arne Seitz,
Scientific Officer
Advanced Light Microscopy
Facility
EMBL
+49 6221 387 8467
Von:
Gesendet: Freitag, 11. Januar 2008
15:20
An: [hidden email]
Betreff: Visitech/Metamorph
confocal
Has anyone had experience with the Visitech Infinity multipoint
confocal system or the new Metamorph ICS variant? We have been planning
on buying a spinning disk but I am wondering if we should look at this
alternative. Dave
Dr. David Knecht
Department of Molecular
and Cell Biology
Co-head Flow Cytometry
and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of
Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
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