Re: Freezing fluorophores
Posted by
Julian Smith III on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Freezing-fluorophores-tp591414p591415.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalWe aliquot and freeze our dye-conjugated secondaries at -20 (not a
frost-free freezer, but real -20C) all the time. They last for at
least a year under these conditions, and in most cases, several years.
Julian
>Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
>Hi,
>
>What's your opinion about freezing fluorescently conjugated
>antibodies? I know for example that the Qdots are destroyed by
>freezing, but how about others?
>
>/fredrik
>
>
>
>--------------------------------------
>Fredrik Wermeling, PhD-student
>Karolinska Institutet
>Department of Medicine
>Unit of Clinical Allergy Research
>L2:04 Karolinska Hospital
>SE-171 76 Stockholm
>Sweden
>
>phone: +46-8-51776696
>mail:
[hidden email]
>fax: +46-8-335724
>--------------------------------------
--
Julian P.S. Smith III
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)