Re: Freezing fluorophores

Posted by Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Freezing-fluorophores-tp591414p591416.html

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We have the same experience as Julian - fluorescently-tagged antibodies in
2-5 microlitre aliquots frozen at -80 have lasted fine since I bought them
in 2000 with no perceptible increase in background though some decrease in
staining intensity.
cheers,
Rosmeary

Rosemary White                    [hidden email]
CSIRO Plant Industry            ph.     61 (0)2-6246 5475
GPO Box 1600                       fax.     [phone]
Canberra, ACT 2601
Australia



On 18/1/08 3:01 AM, "Julian Smith III" <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We aliquot and freeze our dye-conjugated secondaries at -20 (not a
> frost-free freezer, but real -20C) all the time.  They last for at
> least a year under these conditions, and in most cases, several years.
> Julian
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi,
>>
>> What's your opinion about freezing fluorescently conjugated
>> antibodies? I know for example that the Qdots are destroyed by
>> freezing, but how about others?
>>
>> /fredrik
>>
>>
>>
>> --------------------------------------
>> Fredrik Wermeling, PhD-student
>> Karolinska Institutet
>> Department of Medicine
>> Unit of Clinical Allergy Research
>> L2:04 Karolinska Hospital
>> SE-171 76 Stockholm
>> Sweden
>>
>> phone: [phone]
>> mail: [hidden email]
>> fax: [phone]
>> --------------------------------------
>