http://confocal-microscopy-list.275.s1.nabble.com/HCS-in-vivo-imaging-system-tp591433p591437.html
Thanks a lot George. This is to acknowledge the
receipt of mail. I will go through the suggested links
and write back. Need to keep up with some dead-lines.
>
> M, Tsuji K, Yang M, Jiang P, Moossa AR, Hoffman RM.
> Cancer Res 66:
> 11293, for an example. He has published with the
> Olympus products and
> claims to have been involved in developing the
> FluorVivo
> (
http://www.anticancer.com/FluorVivoBrochure.pdf).
>
> I have not yet seen the Xenogen system in action for
> in vivo
> fluorescence. If you want sensitivity by in vivo
> whole (intact) mouse
> imaging, go with Xenogen and firefly luciferase. If
> you want to be
> cutting edge (maybe bleeding edge), be the first to
> publish on a
> FLuc-mKate BRET model.
>
> If you want to image single cells in lymph nodes in
> live mice at
> microscopy resolution, look at the Olympus IV100
> with stick objective
> lens(es) (hopefully in multiphoton mode), or get
> yourself a
> multiphoton microscope. Not tumor papers, but see
> the reviews by
> Hauser, Shlomchik and Haberman 2007 Nat Rev Immunol
> 7: 499-504, and
> by
>
>
> MD, Parker I. Annu Rev Immunol. 2008 Jan 2; [Epub]
> for starters.
> Multiphoton excitation will enable a lot deeper
> imaging into lymph
> node (or liver, if you can get the lens to it) than
> confocal - though
> mKate expressing or DiD, Cy5 or Cy5.5 labeled cells
> confocal imaging
> might not be too bad (long wavelength light scatters
> less).
>
> If the Pan-a-See-Ya fits your requirements (i.e.
> imaging mice at
> macroscopic scale), and John Fox continues to
> decline to do a demo,
> ask if you can buy it on contingency - that is, if
> it works, you pay
> for it, if it does not, you ship it back (negotiate
> in advance who
> should be for shipping and whether a restocking fee
> is in order -
> after all, nce you've used it, it cannot be sold as
> new).
>
> BD CARV HCS system (Pathway Bioimager) - I've seen
> the one in our
> building used for Fura-2 ratio calcium imaging.
> Looked ok. I just saw
> it in action for live pancreatic islet cell imaging,
> did not touch
> any knobs so can't say how sensitive it is compared
> to other systems
> (and did not see any focus changes, so cannot
> address how confocal it
> is). It was being run for sequential well timelapse
> assays, not HCS,
> so I cannot address how well it works for HCS.
>
> best wishes,
>
> George
>
>
>
> At 04:29 AM 1/20/2008, you wrote:
> >Search the CONFOCAL archive at
>
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> >
> >Dear George,
> >
> >Thanks indeed for elaborate information.
> >
> >Among high end in vivo imaging system, on papers
> >Xenogen did appear very good, especially for deep
> >seated bioluminescence, but I could not find
> reports
> >on imaging of GFP or RFP expressing metastatic
> cells.
> >I am interested on imaging metastasis of GFP or RFP
> >expressing cells in liver and lymphnodes. What is
> your
> >opinion and experience reagrding this application
> with
> >Xenogen system?
> >
> >Among lower end systems, FluorVivo of INDECO
> >BioSystems and Pan-a-See-Ya of Lightools looked
> good.
> >But, I could not get demo of Pan-a See-Ya from John
> >Fox. Do you know any users who have sucessfuly used
> >any of these for imaging metastasis in lymphnodes
> and
> >liver?
> >
> >Do you have any infromation on performance HCS
> system
> >of BD that has in built Nipkow disc based confocal
> >system?
> >
> >Once again thanks a lot for the information.
> >With best regards,
> >Nishigandha Naik
> >
> >--- George McNamara <
[hidden email]>
> wrote:
> >
> > > Search the CONFOCAL archive at
> > >
>
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> > >
> > > Hi Nishigandha,
> > >
> > > As always - get a demo! Really more a question
> of
> > > budget and throughput.
> > >
> > > For in vivo, the Caliper/Xenogen IVIS Spectrum
> looks
> > > very good.
> > >
>
>
http://www.caliperls.com/products/optical-imaging/ivis-spectrum.htm> > >
> > > My colleagues at UM are upgrading our IVIS 200
> to
> > > the Spectrum in a
> > > month. Advantage of the IVIS systems is that
> using
> > > cryogenic
> > > back-illuminated CCD's enables luciferase
> > > bioluminescence imaging
> > > (BLI) in vivo (and ex vivo - extremely valuable
> to
> > > take out the
> > > organs at the end and re-image without the rest
> of
> > > the animal in the
> > > way. also, can image multi-well plates, so can
> test
> > > the performance
> > > of cell lines before putting them in the mouse).
> > > A huge advantage of the Xenogen systems is that
> they
> > > have made the
> > > effort to provide quantitative data for
> > > bioluminescence. They use
> > > photons/second/cm^2/steradian. This enables
> every
> > > paper published
> > > with a Xenogen system to be compared to each
> other
> > > (of course, the
> > > depth of the cells, expression level, and amount
> of
> > > luciferin at the
> > > cells are additional variables). If you get a
> > > non-Xenogen BLI
> > > instrument, make sure the vendor validates the
> same
> > > units. Xenogen
> > > now does something similar for fluorescence
> > > tomography (a trickier issue).
> > >
> > > CRi has been in the in vivo fluorescence field
> for
> > > several years.
> > > Check out their
> > >
http://www.cri-inc.com/products/maestro2.asp> > >
> > > If your budget is on the low end, arrange a demo
> > > from John Fox of
> > > Lightools
> (
http://www.lightools.com/tutorial.htm). I
>
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