Analysing x-z and y-z slices
Posted by
Graham Wright-5 on
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Hello,
I have a Z-stack acquired on a Zeiss LSM 510 META
confocal of a sample in 2 channels (single time point). I would like to be
able to extract the x-z and y-z slices so that I can analyse the fluorescence
intensity across them.
I have Imaris and ImageJ at my disposal. In Imaris
I can get a view of these slices quite nicely and adjust the 'depth' and
position of them using the Ortho Slicer and Oblique Slicer options. However I
don't know how, if it is possible, to extract just these slices as images, which
I can later analyse. Can anyone help. Or, does anyone know how to do it in
ImageJ, or indeed another programme?
Alternatively is there a way to directly analyse a
x-z or y-z frame across a 3D reconstruction, for fluorescence intensity, in
any programme?
Thanks in advance for your help,
Graham
-----
Graham Wright
Microscopy & Imaging
Facility
Temasek Life Sciences Laboratory
1 Research
Link
National University of Singapore
Singapore 117604