Posted by
JOEL B. SHEFFIELD on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Analysing-x-z-and-y-z-slices-tp591684p591686.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalIn imageJ, you can use the Image>Stacks>Reslice command to cut a
vertical slice therough a line in any orientation across the x-y
plane. You can decide how many slices you want in your new volume,
and what the spacing should be.
Joel
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Date sent: Tue, 29 Jan 2008 11:14:16 +0800
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From: Graham Wright <
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Subject: Analysing x-z and y-z slices
To:
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello,
I have a Z-stack acquired on a Zeiss LSM 510 META confocal of a
sample in 2 channels (singletime point). I would like to be able to
extract the x-z and y-z slices so that I can analyse the fluorescence
intensity across them.
I have Imaris and ImageJ at my disposal. In Imaris I can get a view
of these slices quite nicely and adjust the 'depth' and position of
them using the Ortho Slicer and Oblique Slicer options. However I
don't know how, if it is possible, to extract just these slices as
images, which I can later analyse. Can anyone help. Or, does anyone
know how to do it in ImageJ, or indeed another programme?
Alternatively is there a way to directly analyse a x-z or y-zframe
across a 3D reconstruction, for fluorescence intensity, in any
programme?
Thanks in advance for your help,
Graham
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Graham Wright
Microscopy & Imaging Facility
Temasek Life Sciences Laboratory
1 Research Link
National University of Singapore
Singapore 117604
P: +65 6872 8406
M: +65 8256 7916
E:
[hidden email]
W:
http://microscopy.tll.org.sgJoel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail:
[hidden email]
URL:
http://astro.temple.edu/~jbs