Nipkow Nyquist matching

Posted by Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nipkow-Nyquist-matching-tp591688.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We recently demo'd several spinning disk confocal systems.  For Nyquist matching of camera and objective, we saw two different solutions.  In one case, a lens was mounted between the scan head and the microscope so that both excitation and emission went through it.  In the other case, it was mounted between the scan head and the camera so only emission went through.  Can anyone comment on the relative merits of this design decision?  THanks- Dave

Dr. David Knecht    

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)