Re: heat and PSF

Posted by M. van de corput on
URL: http://confocal-microscopy-list.275.s1.nabble.com/heat-and-PSF-tp591728p591729.html

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The psf changes not too much in x and y but increased in Z (RT compared to
37oC). So you will lose resolution mostly in z. This was measuresd with
fixed tetraspeck beads 63x oil objective. I have no idea how the psf
behaves with other objectives or during life imaging. Cooling from 37oC to
RT untill the PSF is stable again took on our system about 2.5 to 3
hours.(all done with the meta LSM 510).


Mariette

____________________________________________

Dr. M.P.C. Kemner-van de Corput
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MGC - Dept. of Cell Biology & Genetics
Erasmus Medical Center
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Op Do, 7 februari, 2008 7:57 pm, schreef Carl Boswell:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
> Does anyone have experience with characterizing PSF's (or other aspects of
> the optics) at different temperatures?  For instance, how much effect  is
> there on an objective when the temp is raised from room temp (25C) to
> physiologically relevant 37C?
> Thanks,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX [phone]
>