Re: heat and PSF

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/heat-and-PSF-tp591728p591731.html

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The difference will come with the SA correction.  TIRF
lenses (at least the Leica one) actually have values for
different temperatures on the correction ring.  This is
working with oil, which changes RI rather a lot with
temperature.  In water the difference will be smaller, but
it's still worth setting the correction collar visually
rather than by trusting the scale.  Once you have set it
correctly there should be no real difference in PSF.

                                              Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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     http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Carl Boswell
Sent: Friday, 8 February 2008 5:58 AM
To: [hidden email]
Subject: heat and PSF

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,
Does anyone have experience with characterizing PSF's (or other aspects of the optics) at different temperatures?  For instance, how much effect  is there on an objective when the temp is raised from room temp (25C) to physiologically relevant 37C?
Thanks,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
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