http://confocal-microscopy-list.275.s1.nabble.com/DIC-phase-through-spinning-disk-tp591738p591748.html
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> Well, I'm baffled. The QPm approach uses defocus contrast to calculate
> phase shift. Confocal imaging should eliminate this. So I can't see how
> this could work.
>
>
> Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
>
http://www.guycox.com/optical.htm> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
>
http://www.guycox.net
> <
https://www.mcws.usyd.edu.au/exchweb/bin/redir.asp?URL=https://www.mcws.usyd.edu.au/exchweb/bin/redir.asp?URL=http://www.guycox.net>
>
> ------------------------------------------------------------------------
> *From:* Confocal Microscopy List on behalf of Stephen Cody
> *Sent:* Tue 08/01/29 11:29 AM
> *To:*
[hidden email]
> *Subject:* Re: DIC/phase through spinning disk
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Search the
> CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> G’day David,
>
>
>
> While I have not tried the following technique with spinning disk
> confocals, I have used it with brightfield images collected with
> “conventional point scanning” confocals, I think it should also work
> well with spinning disk systems.
>
>
>
> If you have a “through focus” series of brightfield images you can
> calculate the DIC images (or phase, Hoffman etc, etc) using software
> called QPm (Or QPI) Developed by Keith A. Nugent et al., and supplied by
> iatia. See list of papers at www.iatia.com <
http://www.iatia.com/> .
> You should be able to do this with any archival data you have saved to
> disk, provided it is a z-series of brightfield images.
>
>
>
> You can see my paper specifically relating to QPm and confocal:
>
> Cody, S.H., Xiang, S.D., Layton, M.J., Handman, E., Lam, M.H.C., Nice,
> E.C., and Heath, J.K. A simple method allows DIC imaging in conjunction
> with confocal microscopy. J. Microscopy. 217: 265-274 (2005).
>
>
http://dx.doi.org/10.1111/j.1365-2818.2005.01452.x>
>
>
> Application note:
>
>
http://www.iatia.com.au/technology/applicationNotes/qpmApplicationWithConfocalMicroscopes.pdf
>
>
>
>
> Other application notes:
>
>
http://www.iatia.com.au/technology/applicationNotes.asp>
>
>
> Cheers
>
> *Stephen H. Cody*
> Microscopy Manager
> *C*entral* R*esource for* A*dvanced* M*icroscopy
> Ludwig Institute for Cancer Research
> PO Box 2008 Royal Melbourne Hospital
> Victoria, 3050
> Australia
> *Tel:* 61 3 9341 3155 * **Fax:* 61 3 9341 3104
> *email:*
[hidden email] <mailto:
[hidden email]>
> www.ludwig.edu.au/labs/confocal.html
> <
http://www.ludwig.edu.au/labs/confocal.html>
> www.ludwig.edu.au/confocal <
http://www.ludwig.edu.au/confocal>
>
> Tip: Learn how to receive reminders about you microscope booking:
> www.ludwig.edu.au/confocal/Local/Booking_Hint.htm
> <
http://www.ludwig.edu.au/confocal/Local/Booking_Hint.htm>
>
> -----Original Message-----
> *From:* Confocal Microscopy List [mailto:
[hidden email]]
> *On Behalf Of *David Knecht
> *Sent:* Thursday, 10 January 2008 11:58 PM
> *To:*
[hidden email]
> *Subject:* DIC/phase through spinning disk
>
>
>
> We are in the process of putting together a spinning disk confocal
> system. We have heard mixed things about how acceptable it is to do
> transmitted light microscopy (DIC or Phase) through the spinning disk,
> as opposed to running a separate camera through a separate microscope
> port. What have others found?
>
>
>
> Dr. David Knecht
>
> Department of Molecular and Cell Biology
>
> Co-head Flow Cytometry and Confocal Microscopy Facility
>
> U-3125
>
> 91 N. Eagleville Rd.
>
> University of Connecticut
>
> Storrs, CT 06269
>
> 860-486-2200
>
> 860-486-4331 (fax)
>
>
>
>
>
> ------------------------------------------------------------------------
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