Dear David et al.,
In regards to the question about DIC and
spinning disk confocals. I would like to point to the versatile design of the
Olympus DSU spinning disk confocal system. With the DSU, the disk is
easily moved in and out of the optical path via software, providing an elegant
solution to this issue. You can easily configure the system to acquire fluorescent
images and brightfield images with or without the disk. Therefore, you
utilize the same optical path and the same camera for brightfield, confocal and
epifluorescent imaging. This makes acquiring the highest quality fluorescent/DIC
overlays effortless. Additionally, there is no requirement for a second
camera, which alleviates any issues with aligning/registering two cameras. Additionally,
registering a second camera could be either impossible or very expensive given
the options for cameras with the same sensor dimensions as the commonly
specified EMCCDs. Furthermore, the ease of switching the disk in and out
of the optical path makes the DSU increasingly more versatile. For
example, the DSU also is a fully functional epifluorescent scope and can be
used such with or without deconvolution, or can be used in combination with
filter wheels for Ca2+ imaging etc. This allows one scope to be used for multiple
purposes, maximizing the expenses invested in high end frames and cameras.
For those requiring the utmost in live
cell imaging, by alleviating the need for second cameras, or entire imaging systems,
there should be plenty of room in the budget for the Olympus IX81-ZDC; assuring
the proper focal plane is maintained during your long-term time lapse
acquisitions.
Sincerely,
For those interested in seeing any Olympus
systems in the
J.D. Herlihy,
Ph.D.
Research and
Imaging Specialist
Optical
Analysis Corporation
800-588-6054
Cell:
508-965-8894
From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of David Knecht
Sent: Thursday, January 10, 2008
7:58 AM
To: [hidden email]
Subject: DIC/phase through
spinning disk
We are in the process of putting together a spinning disk confocal
system. We have heard mixed things about how acceptable it is to do
transmitted light microscopy (DIC or Phase) through the spinning disk, as
opposed to running a separate camera through a separate microscope port.
What have others found?
Dr. David Knecht
Department of Molecular
and Cell Biology
Co-head Flow Cytometry
and Confocal Microscopy Facility
U-3125
860-486-2200
860-486-4331 (fax)
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