Re: DIC/phase through spinning disk

Posted by Paul Maddox on
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Hi Dave,

All you need is a shutter for transmitted light and an emission filter
wheel between the CSU head and the camera for DIC.  If you put the
analyzer in the filter wheel aligned properly to the polarizer (crossed)
you can make excellent DIC images by rotating the analyzer in and out of
the imaging path.  See Maddox, et al. 2003. A spinning disk confocal
microscope system for rapid high resolution, multimode, fluorescence
speckle microscopy and GFP imaging in living cells. In, Biophotonics,
Parts, A and B (G. Marriot and I. Parker, eds.), Methods in Enyzmology,
Vol. 360, Academic Press, SanDiego, CA.  for more details.

Paul