Posted by
Gary Laevsky-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIC-phase-through-spinning-disk-tp591738p591755.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Paul,
Although you can get excellent DIC images through the disc (using the
very easy mechanism you state), there is some artifact introduced by the
disc.
Best,
Gary Laevsky, Ph.D.
Imaging Application Specialist
Andor Technology
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Paul S. Maddox
Sent: Thursday, January 10, 2008 12:12 PM
To:
[hidden email]
Subject: Re: DIC/phase through spinning disk
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Dave,
All you need is a shutter for transmitted light and an emission filter
wheel between the CSU head and the camera for DIC. If you put the
analyzer in the filter wheel aligned properly to the polarizer (crossed)
you can make excellent DIC images by rotating the analyzer in and out of
the imaging path. See Maddox, et al. 2003. A spinning disk confocal
microscope system for rapid high resolution, multimode, fluorescence
speckle microscopy and GFP imaging in living cells. In, Biophotonics,
Parts, A and B (G. Marriot and I. Parker, eds.), Methods in Enyzmology,
Vol. 360, Academic Press, SanDiego, CA. for more details.
Paul