Re: DIC/phase through spinning disk
Posted by
Knecht, David on
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Yes, except if I remember correctly, that option costs more than a second camera, so I'm not sure that is the best option. I would rather have a second camera for the same cost. The downside is alignment of the two images if it is separate cameras. Dave
On Jan 10, 2008, at 8:41 AM, Gary Laevsky wrote:
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Dave,
With the CSUX, there is a disc bypass option, so with the correct optics in the train, you can get true clean DIC/phase with one camera.
From: Confocal Microscopy List [[hidden email]] On Behalf Of David Knecht
Sent: Thursday, January 10, 2008 7:58 AM
To: [hidden email]
Subject: DIC/phase through spinning disk
We are in the process of putting together a spinning disk confocal system. We have heard mixed things about how acceptable it is to do transmitted light microscopy (DIC or Phase) through the spinning disk, as opposed to running a separate camera through a separate microscope port. What have others found?
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
University of Connecticut
Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)