Confocal Microscopy List

Re: Frap analysis

Posted by Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Frap-analysis-tp591758p591764.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Anchal,

Perhaps you could have a look at the review articles referenced below, and see among the references if there are any that deal with work similar to what you plan to do; thwe first two, you should be able to look up in PubMed and download for free.

Carrero G, McDonald D, Crawford C, et al. Using FRAP and mathematical modeling to determine the in vivo kinetics of nuclear proteins. Methods 29: 14-28 (2003).

Lippincott-Schwartz J, Altan_Bonnet N, Patterson G. Photobleaching and photoactivation: following protein dynamics in living cells. Nature Reviews S7-S14 (2003) (check Medline for exact reference)

and this Chapter in Live Cell Imaging: A Laboratory Manual. CSHL Press, Goldman and Spector (Eds):

G Rabut and J Ellenberg: Photobleaching Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, iFRAP, and FLIP. Ch 7, pp 101-126. Lots of useful references there...

That could be a start...

Julio Vazquez, PhD

Fred Hutchinson Cancer Research Center

1100 Fairview Ave. N.,

Seattle, WA 98109-1024