Re: A1

Posted by Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FW-A1-tp591961p591962.html

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The ability of using this system along with Controlled Light Exposure
Microscopy (CLEM) makes this system for long time live cell imaging and
confocal analysis. CLEM automatically monitors and varying the laser
illumination during time-lapse studies to reduce the risk of laser induced
bleaching, biochemical inconsistency and cell death.

Can you clarify what this means?  I don't see how you can monitor the imaging and vary the laser in a way that can avoid cell toxicity.  Typically, you don't want the laser intensity to vary.  Do you have any quantitative data on cellular toxicity during imaging in comparison to a spinning disk system?

Dr. David Knecht    

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)