Re: A1

Posted by lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FW-A1-tp591961p591969.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You may want to read this article, which idea is implemented by Nikon
for their system:

Hoebe RA, Van Oven CH, Gadella TW Jr, Dhonukshe PB, Van Noorden CJ, Manders EM.
Controlled light-exposure microscopy reduces photobleaching and
phototoxicity in fluorescence live-cell imaging.
Nat Biotechnol. 2007 Feb;25(2):249-53. Epub 2007 Jan 21.

Christophe Leterrier

On Feb 18, 2008 4:50 PM, David Knecht <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> The ability of using this system along with Controlled Light Exposure
> Microscopy (CLEM) makes this system for long time live cell imaging and
> confocal analysis. CLEM automatically monitors and varying the laser
> illumination during time-lapse studies to reduce the risk of laser induced
> bleaching, biochemical inconsistency and cell death.
>
> Can you clarify what this means?  I don't see how you can monitor the
> imaging and vary the laser in a way that can avoid cell toxicity.
> Typically, you don't want the laser intensity to vary.  Do you have any
> quantitative data on cellular toxicity during imaging in comparison to a
> spinning disk system?
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>