Interaction PFA vs rhodamine

Posted by Louis Villeneuve on
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Bonjour à Tous!

We are labeling the endothelium of an UNfixed blood vessel with a polymer conjugated with Rhodamine and then, fix the blood vessel with fresh paraformaldehyde (PFA) 4% (ph 7.4).  Does the PFA  could interact with the Rhodamine and affect the staining?

Thanks a lot for your help!

Louis

Louis Villeneuve
Research Associate- Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

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