Re: Interaction PFA vs rhodamine

Posted by Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Interaction-PFA-vs-rhodamine-tp592004p592005.html

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hello Louis,
Rhodamine conjugated to fixable dextrans has been fully compatible  
with 4% pfa for our work with brain tissue.  in zebrafish the  
autofluorescence is horrendous under widefield fluorescence, but much  
reduced by confocal.

Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

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On May 8, 2008, at 6:19 AM, Louis Villeneuve wrote:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi- 
> bin/wa?S1=confocal
> Bonjour à Tous!
>
> We are labeling the endothelium of an UNfixed blood vessel with a  
> polymer conjugated with Rhodamine and then, fix the blood vessel  
> with fresh paraformaldehyde (PFA) 4% (ph 7.4).  Does the PFA  could  
> interact with the Rhodamine and affect the staining?
>
> Thanks a lot for your help!
>
> Louis
>
> Louis Villeneuve
> Research Associate- Confocal Microscopy
> Heart Montreal Institute- Research Center
> 5000 East Belanger
> Montreal (Qc), Canada
> H1T 1C8
>
> 514-376-3330 ext 3511
> 514-376-1355 (Fax)
>
> [hidden email]
>