Preparation question, not microscopy related

Posted by Farid Jalali on
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Hello All,

Does anyone have experience storing coverslips that have been fixed with freshly prepared 2% paraformaldehyde for long term, say a month or so? I would eventually label them for indirect immunofluorescence using primary antibodies and fluorophore-conjugated secondary antibodies? This is not a standard procedure for me but circumstances require me to consider this if possible. Any advice or suggestions would be greatly appreciated.

Cheers

Farid

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Farid Jalali MSc
Senior Research Technician- Lab Manager
Applied Molecular Oncology/ Princess Margaret Hospital
STTARR Innovation Facility/ Radiation Medicine Program
 

Toronto, Canada
 

416-946-4501 X4351 (Princess Margaret Hospital)
416-581-7754 STTARR at MaRS Building
416-581-7791 STTARR Microscopy Suite