Re: Preparation question, not microscopy related

Posted by Masur, Sandra on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Preparation-question-not-microscopy-related-tp592015p592016.html

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Yes - but after fixation, briefly permeabilize with dilute Triton  
X-100 and then rinse before putting back in PBS. This process will  
decrease the potential for cellular structre degradation by lightly  
fixed and still active enzymes.


On Jun 9, 2008, at 10:09 AM, Farid Jalali wrote:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi- 
> bin/wa?S1=confocal
> Hello All,
> Does anyone have experience storing coverslips that have been fixed  
> with freshly prepared 2% paraformaldehyde for long term, say a  
> month or so? I would eventually label them for indirect  
> immunofluorescence using primary antibodies and fluorophore-
> conjugated secondary antibodies? This is not a standard procedure  
> for me but circumstances require me to consider this if possible.  
> Any advice or suggestions would be greatly appreciated.
> Cheers
> Farid
>
> --
> Farid Jalali MSc
> Senior Research Technician- Lab Manager
> Applied Molecular Oncology/ Princess Margaret Hospital
> STTARR Innovation Facility/ Radiation Medicine Program
>
> Toronto, Canada
>
> 416-946-4501 X4351 (Princess Margaret Hospital)
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> 416-581-7791 STTARR Microscopy Suite