Re: Preparation question, not microscopy related
Posted by
lechristophe on
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Usually when I store my PFA-fixed cells for more than overnight I fix with 4% PFA,rinse, and put back in 0.1% PFA to avoid growth of contaminants that can occur even if stored at 4°C. An alternative is to use sodium azide, but it is simpler to just dilute the fixative and works very well in my hands.
Christophe
On Mon, Jun 9, 2008 at 16:41, Sandra Masur <
[hidden email]> wrote:
Yes - but after fixation, briefly permeabilize with dilute Triton X-100 and then rinse before putting back in PBS. This process will decrease the potential for cellular structre degradation by lightly fixed and still active enzymes.
On Jun 9, 2008, at 10:09 AM, Farid Jalali wrote:
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Hello All,
Does anyone have experience storing coverslips that have been fixed with freshly prepared 2% paraformaldehyde for long term, say a month or so? I would eventually label them for indirect immunofluorescence using primary antibodies and fluorophore-conjugated secondary antibodies? This is not a standard procedure for me but circumstances require me to consider this if possible. Any advice or suggestions would be greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician- Lab Manager
Applied Molecular Oncology/ Princess Margaret Hospital
STTARR Innovation Facility/ Radiation Medicine Program
Toronto, Canada
416-946-4501 X4351 (Princess Margaret Hospital)
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