http://confocal-microscopy-list.275.s1.nabble.com/DNA-dyes-tp592122p592128.html
were just in the short term (a couple of hours). I've not managed to get
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Carl, Charles,
>
> The response Charles described is to be expected using Hoechst, if UV
> (=< 405 nm) excitation is used. Same goes for DAPI, with the
> additional problem that DAPI requires permeabilized cells making it
> unsuitable for most live cell experiments.
>
> I have not tried this but I am sure someone out there probably has,
> namely, multi-photon confocal either 2 P or 3 P to excite Hoechst,
> which is PM permeable. UV by itself will elicit radical generation
> and strand breaking. Hoechst being a minor groove DNA dye could lead
> to a lower risk of scission when using 2 P or 3 P to get excitation,
> and might pose less risk of collateral DNA damage including unwanted
> crosslinking.
>
> Further, I would be interested to know whether Hoechst added to cells
> in culture with no light excitation and maintained through what would
> be a couple of passages would permit any cell division. I.E., does
> the dye+light abolish cell division or dye by itself?
>
> Regards All,
> Mario
>
> PS I know that MP illumination can really cook cells, too. Guys what
> about using DRAQ5 to watch mitosis?
>
>
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>
>> Dear all,
>> What is the consensus on using either DAPI or Hoechst staining on
>> live cells? Are there effects on mitosis and/or chromosome movement?
>> Thanks,
>>
>> Carl
>>
>> Carl A. Boswell, Ph.D.
>> Molecular and Cellular Biology
>> University of Arizona
>> 520-954-7053
>> FAX 520-621-3709
>