Re: DNA dyes
Posted by
Julio Vazquez on
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Carl,
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I suspect that UV, more than the dye itself, is toxic to cells and to DNA. In addition to DNA dyes with excitation in the visible range, you could, if possible, use a Histone-GFP chromatin label. The Histone-GFP system has been used successfully in several systems for this purpose, and the fusion protein by itself must have very low toxicity, if any, as transformed fly lines carrying it have been around for years now. I have followed Drosophila embryos through several nuclear/cell division cycles with this system, and those embryos can keep on developing. In any event, it would be good to have a biological test (cell viability, cell division, continuation of development) to make sure your imaging regime is not having adverse effects on your specific question.
Julio.
On Mar 12, 2008, at 12:23 PM, Carl Boswell wrote:
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Dear all,
What is the consensus on using either DAPI or Hoechst staining on live cells? Are there effects on mitosis and/or chromosome movement?
Thanks,
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709