Re: Syto dyes for plant nuclei in living cells
Posted by
Tobias Baskin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592150.html
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Re: Syto dyes for plant nuclei in living
cells
John,
One or
two years ago, we got a group of syto dyes for this very purpose,
imaging in arabidopsis roots. We were hoping to image DNA along with a
GFP reporter and therefore we tested sytos that have long wavelength
emission (we were working on confocal, without ability to do uv
excitation). None of them worked for us. They tended to be either
toxic or dim. The other thing we noticed was that because the cells
are crammed with mitochondria that move all over the place, it was
difficult to follow nuclear DNA against this hive of mitochondrial
motion. But we never tested blue ones.
Good
luck,
Tobias
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Do any of the plant biologists out there have experience using the
Syto dyes from Molecular Probes to stain nuclei of living cells.
In particular, they list 5 different dyes in the blue emission range
(Syto 40-45). Syto 42 looks good to me on paper. What do
you think. Thanks, John.
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