Re: Syto dyes for plant nuclei in living cells

Posted by Christian-103 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592151.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I'll echo Tobias here.  It's very tough to get any dye into living plant cells and keep them that way.  We've tried nearly every nuclear stain available along with various other markers and trackers, such as mitotracker, all with no luck in the hands of the users.

Have you thought about making stables with RFP in the nuclei?  Three channel sequencing then allows you to visualize GFP, RFP and cholorplasts in the far red channel. 

Christian