Re: Syto dyes for plant nuclei in living cells

Posted by Christian-103 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592155.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Quick and dirty... hmm, might I suggest animal cells then? 

Yes, I'm joking.  I've done primarily plant confocal since 2001, and I don't know anything quick and dirty yet, but I'm always hopeful.

Maybe a few of the other responses here will show some promise.  I will say what works in one species may not in another, no matter how much time you throw at it.

If you have success, please share it here.

Christian


John Runions <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Thanks Christian,  good suggestion about using FPs.  We already have almost every organelle marked in almost every color and that clearly is the way to go.  We were only interested in nuclear stains that emit in the blue as a 'quick and dirty' way of looking at nuclei in lines without nuclear marking by FPs.  Best, John.

Christian wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I'll echo Tobias here.  It's very tough to get any dye into living plant cells and keep them that way.  We've tried nearly every nuclear stain available along with various other markers and trackers, such as mitotracker, all with no luck in the hands of the users.

Have you thought about making stables with RFP in the nuclei?  Three channel sequencing then allows you to visualize GFP, RFP and cholorplasts in the far red channel. 

Christian