Re: Syto dyes for plant nuclei in living cells
Posted by
Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592156.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Re: Syto dyes for plant nuclei in living cells
Oh yes, I forgot to mention that we also found a few to be toxic (had forgotten....). We tried the green and orange ones.
cheers,
Rosemary
On 8/5/08 11:03 PM, "Tobias Baskin" <[hidden email]> wrote:
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
John,
One or two years ago, we got a group of syto dyes for this very purpose, imaging in arabidopsis roots. We were hoping to image DNA along with a GFP reporter and therefore we tested sytos that have long wavelength emission (we were working on confocal, without ability to do uv excitation). None of them worked for us. They tended to be either toxic or dim. The other thing we noticed was that because the cells are crammed with mitochondria that move all over the place, it was difficult to follow nuclear DNA against this hive of mitochondrial motion. But we never tested blue ones.
Good luck,
Tobias
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Do any of the plant biologists out there have experience using the Syto dyes from Molecular Probes to stain nuclei of living cells. In particular, they list 5 different dyes in the blue emission range (Syto 40-45). Syto 42 looks good to me on paper. What do you think. Thanks, John.
--
Runions signature
*********************************
C. John Runions, Ph.D.
School of Life Sciences
Oxford Brookes University
Oxford, UK
OX3 0BP
email: [hidden email]
phone: +44 (0) 1865 483 964
Runions' lab web site <http://www.brookes.ac.uk/lifesci/runions/HTMLpages/index.html%21>
Visit The Illuminated Plant Cell <http://www.illuminatedcell.com/ER.html> dot com
Oxford Brookes Master's in Bioimaging with Molecular Technology <http://www.brookes.ac.uk/studying/courses/postgraduate/2007/bmt>