Re: Syto dyes for plant nuclei in living cells

Posted by Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592159.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi John,

I tried a whole range of them at one stage, but in my hands, they all labelled quite a bit more than the nucleus, some just went in and lit up the whole cytoplasm.  Didn’t pursue it further than that, it may be that with different buffers, etc. they would be less likely to rush into the cell.
cheers,
Rosemary

Rosemary White                    [hidden email]
CSIRO Plant Industry            ph.     61 (0)2-6246 5475
GPO Box 1600                       fax.     [phone]
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Australia



On 8/5/08 8:13 PM, "John Runions" <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Do any of the plant biologists out there have experience using the Syto dyes from Molecular Probes to stain nuclei of living cells.  In particular, they list 5 different dyes in the blue emission range (Syto 40-45).  Syto 42 looks good to me on paper.  What do you think. Thanks, John.