Re: Syto dyes for plant nuclei in living cells

Posted by Christian-103 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592160.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I'd like to point out that three researchers on three continents have now stated the difficulty getting only the nuclei labeled.  I know we had some input from a commercial source, but the literature is lacking.

Nearly every day I'm asked "What's that spotting in the cytoplasm?"  And I answer "I don't know without a counter stain."

The point I'm trying to make is that plant confocal is challenging, and none of us are likely to get bored any time soon.  Now if we could just have 1/10th the dyes the animal systems people do.

I'd also like to make a public plea for any protocols which may lead to nuclei only labeling in living plants.

Christian