Re: Syto dyes for plant nuclei in living cells

Posted by Marc Green on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Syto-dyes-for-plant-nuclei-in-living-cells-tp592149p592161.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal (another Red-not-blue and not in plant comment)

I tried several concentrations and incubation times for the syto dyes in the red sampler kit on live yeast,
But never got any specific nuclear staining. Sometimes the opposite.
I was watching on both “Texas-Red” and “Cy5” channels.

Please let me know if you get a working blue past the cell wall at a non-toxic concentration.

Marc

On 8/5/08 03:13, "John Runions" <[hidden email]> wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Do any of the plant biologists out there have experience using the Syto dyes from Molecular Probes to stain nuclei of living cells.  In particular, they list 5 different dyes in the blue emission range (Syto 40-45).  Syto 42 looks good to me on paper.  What do you think. Thanks, John.

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Marc D Green
Pombe.net
[hidden email]