Not a confocal question

Posted by Matthew Pearson-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Not-a-confocal-question-tp592323.html

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This is not specifically a confocal question but a microscopy question none the less.  We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.  There seems to be an image artifact somewhere in the optical train.  When I have the live camera feed there are lots of small circles with smaller rings inside.  Could this be dust that has collected somewhere? I have tried cleaning every surface I can get access to but it is still there! I get it whether doing bright field or fluorescence and it is not dirty objective lenses.  It is not filters within the condenser either as the same particles appear in the image irrespective of the condenser position.  I have removed the camera and cleaned it, still no look.  So I'm at a loose end really as to what it can be.  I can only think it is something within the microscope body beneath the objective housing and I don't really want to dismantle the whole thing! Has anyone had similar problems in the past?

Thanks,

Matt Pearson.