Posted by
Rittling, Susan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Not-a-confocal-question-tp592323p592324.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalWe had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing defect)
and he replaced it for free.
Maybe it it's the same problem with your camera? Try contacting your
sales rep.
Susan R Rittling
The Forsyth Institute
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To:
[hidden email]
Subject: Not a confocal question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
<html>
<head>
</head>
<body bgcolor="#ffffff" text="#000000">
<br>
This is not specifically a confocal question but a microscopy question
none the less. We have a Zeiss axiovert 100M using a Hamamatsu
Orca
camera. There seems to be an image artifact somewhere in the
optical
train. When I have the live camera feed there are lots of small
circles with smaller rings inside. Could this be dust that has
collected somewhere? I have tried cleaning every surface I can get
access to but it is still there! I get it whether doing bright field or
fluorescence and it is not dirty objective lenses. It is not
filters
within the condenser either as the same particles appear in the image
irrespective of the condenser position. I have removed the camera
and
cleaned it, still no look. So I'm at a loose end really as to what
it
can be. I can only think it is something within the microscope
body
beneath the objective housing and I don't really want to dismantle the
whole thing! Has anyone had similar problems in the past? <br>
<br>
Thanks,<br>
<br>
Matt Pearson.<br>
<div class="moz-signature">
<title></title>
<br>
<div class="moz-signature">
<div class="moz-signature"><br>
<pre class="moz-signature" cols="72"><font color="#3366ff"><font
color="#3333ff"><font face="Arial">--
Imaging Technician
Cell Biology Division
Institute of Ophthalmology
University College London
EC1V 9EL
020 7608 6857
<a class="moz-txt-link-abbreviated"
href="mailto:
[hidden email]">
[hidden email]</a></f
ont></font><font color="#3333ff">
</font>
</font></pre>
</div>
</div>
</div>
</body>
</html>