Hi!
There was someone speaking about a similar
issue with stripes and circles as imaging “artifacts” recently and
they mentioned that condensation might also be an issue.
Kind regards,
Deanne.
Deanne Catmull
Fax: 9341-1597
Ph: 9341-1577
From:
Sent: Thursday, March 13, 2008
6:54 AM
To: [hidden email]
Subject: Re: Not a confocal
question
Matt, we had same problems in two IX-70s one is with Photometrics Sensys camera
and other one is coupled with a Trans light PMT detector in a Biorad MRC 2000
confocal system!. I remember we did exactly all those tests suggested by others
in this thread and figured out in both cases it is on the detector side.
Shiv
At 01:12 PM 3/12/2008, you wrote:
Search the CONFOCAL
archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
We had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing defect)
and he replaced it for free.
Maybe it it's the same problem with your camera? Try contacting your
sales rep.
Susan R Rittling
The Forsyth Institute
-----Original Message-----
From:
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To: [hidden email]
Subject: Not a confocal question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
This is not specifically a confocal question but a microscopy question none the
less. We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.
There seems to be an image artifact somewhere in the optical train. When
I have the live camera feed there are lots of small circles with smaller rings
inside. Could this be dust that has collected somewhere? I have tried
cleaning every surface I can get access to but it is still there! I get it
whether doing bright field or fluorescence and it is not dirty objective
lenses. It is not filters within the condenser either as the same particles
appear in the image irrespective of the condenser position. I have
removed the camera and cleaned it, still no look. So I'm at a loose end
really as to what it can be. I can only think it is something within the
microscope body beneath the objective housing and I don't really want to
dismantle the whole thing! Has anyone had similar problems in the past?
Thanks,
Matt Pearson.
-- Imaging TechnicianCell Biology Division