Re: Not a confocal question
Posted by
Barbara Foster on
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Just a reminder that diffraction is active in this sort of imaging and it
is always true that light, bending around the edges of very small
features, will undergo diffraction and, subsequently, interference.
It is that alternating pattern of constructive destructive interference
that causes the finges or bullseye type of patterns. These are,
indeed, artifacts, and the cleaner you can keep your optical system, the
less you will see them.
Good hunting!
Barbara Foster, President
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At 01:11 AM 3/26/2008, you wrote:
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Hi!
There was someone speaking about a similar issue with stripes and circles
as imaging “artifacts” recently and they mentioned that condensation
might also be an issue.
Kind regards,
Deanne.
Deanne
Catmull Research Assistant
School of Dental Science
University of Melbourne
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From: Confocal Microscopy List
[[hidden email]] On Behalf Of Mayandi
Sivaguru
Sent: Thursday, March 13, 2008 6:54 AM
To: [hidden email]
Subject: Re: Not a confocal question
Matt, we had same problems in two IX-70s one is with Photometrics Sensys
camera and other one is coupled with a Trans light PMT detector in a
Biorad MRC 2000 confocal system!. I remember we did exactly all those
tests suggested by others in this thread and figured out in both cases it
is on the detector side.
Shiv
At 01:12 PM 3/12/2008, you wrote:
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We had the same frustrating experience with an AxioCam HrC camera. I
tried to clean everything and concluded that the problem was inside
the
camera. I finally contacted the Zeiss sales rep, and he replied that
many of these cameras have this problem (maybe a manufacturing
defect)
and he replaced it for free.
Maybe it it's the same problem with your camera? Try contacting
your
sales rep.
Susan R Rittling
The Forsyth Institute
-----Original Message-----
From: Confocal Microscopy List [
[hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008 12:29 PM
To: [hidden email]
Subject: Not a confocal question
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This is not specifically a confocal question but a microscopy question
none the less. We have a Zeiss axiovert 100M using a Hamamatsu Orca
camera. There seems to be an image artifact somewhere in the
optical train. When I have the live camera feed there are lots of
small circles with smaller rings inside. Could this be dust that
has collected somewhere? I have tried cleaning every surface I can get
access to but it is still there! I get it whether doing bright field or
fluorescence and it is not dirty objective lenses. It is not
filters within the condenser either as the same particles appear in the
image irrespective of the condenser position. I have removed the
camera and cleaned it, still no look. So I'm at a loose end really
as to what it can be. I can only think it is something within the
microscope body beneath the objective housing and I don't really want to
dismantle the whole thing! Has anyone had similar problems in the past?
Thanks,
Matt Pearson.
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Mayandi Sivaguru, PhD,
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