Not sure if this will help but had a problem with a ghost image that turned out to be a messed up calibration. Just do a Black and shading reference. If the problem persist, I agree, contact
Zeiss.
Stephan
EMU
University of Botswana
Gaborone
Botswana
-----Original Message-----
From: Confocal Microscopy List on behalf of Mayandi Sivaguru
Sent: Wed 3/12/2008 9:54 PM
To: [hidden email]
Subject: Re: Not a confocal question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Matt, we had same problems in two IX-70s one is with Photometrics
Sensys camera and other one is coupled with a Trans light PMT
detector in a Biorad MRC 2000 confocal system!. I remember we did
exactly all those tests suggested by others in this thread and
figured out in both cases it is on the detector side.
Shiv
At 01:12 PM 3/12/2008, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We had the same frustrating experience with an AxioCam HrC camera. I
>tried to clean everything and concluded that the problem was inside the
>camera. I finally contacted the Zeiss sales rep, and he replied that
>many of these cameras have this problem (maybe a manufacturing defect)
>and he replaced it for free.
>Maybe it it's the same problem with your camera? Try contacting your
>sales rep.
>
>Susan R Rittling
>The Forsyth Institute
>
>-----Original Message-----
>From: Confocal Microscopy List [[hidden email]] On
>Behalf Of Matthew Pearson
>Sent: Wednesday, March 12, 2008 12:29 PM
>To: [hidden email]
>Subject: Not a confocal question
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
><!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
>
>This is not specifically a confocal question but a microscopy
>question none the less. We have a Zeiss axiovert 100M using a
>Hamamatsu Orca camera. There seems to be an image artifact
>somewhere in the optical train. When I have the live camera feed
>there are lots of small circles with smaller rings inside. Could
>this be dust that has collected somewhere? I have tried cleaning
>every surface I can get access to but it is still there! I get it
>whether doing bright field or fluorescence and it is not dirty
>objective lenses. It is not filters within the condenser either as
>the same particles appear in the image irrespective of the condenser
>position. I have removed the camera and cleaned it, still no
>look. So I'm at a loose end really as to what it can be. I can
>only think it is something within the microscope body beneath the
>objective housing and I don't really want to dismantle the whole
>thing! Has anyone had similar problems in the past?
>
>Thanks,
>
>Matt Pearson.
>
>
>
>--
>Imaging Technician
>Cell Biology Division
>Institute of Ophthalmology
>University College London
>EC1V 9EL
>020 7608 6857
><[hidden email]>[hidden email]
>
>
Mayandi Sivaguru, PhD, PhDMicroscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA
Office: 217.333.1214
Fax: 217.244.2496
[hidden email]
http://core.igb.uiuc.edu
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