http://confocal-microscopy-list.275.s1.nabble.com/Not-a-confocal-question-tp592323p592331.html
cooled CCD. If you want to test for it without opening the camera,
immediately after switching it on again. If the pattern is gone and
>Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>This is not specifically a confocal question but a microscopy
>question none the less. We have a Zeiss axiovert 100M using a
>Hamamatsu Orca camera. There seems to be an image artifact
>somewhere in the optical train. When I have the live camera feed
>there are lots of small circles with smaller rings inside. Could
>this be dust that has collected somewhere? I have tried cleaning
>every surface I can get access to but it is still there! I get it
>whether doing bright field or fluorescence and it is not dirty
>objective lenses. It is not filters within the condenser either as
>the same particles appear in the image irrespective of the condenser
>position. I have removed the camera and cleaned it, still no
>look. So I'm at a loose end really as to what it can be. I can
>only think it is something within the microscope body beneath the
>objective housing and I don't really want to dismantle the whole
>thing! Has anyone had similar problems in the past?
>
>Thanks,
>
>Matt Pearson.
>
>
>
>
>--
>Imaging Technician
>Cell Biology Division
>Institute of Ophthalmology
>University College London
>EC1V 9EL
>020 7608 6857
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