Re: Not a confocal question
Posted by
Ian Dobbie-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Not-a-confocal-question-tp592323p592333.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalRussell Spear <
[hidden email]> writes:
> Can you rotate the camera? If the "dirt" moves it with the camera or
> the mount. I it stays in same place it's somewhere in the microscope
> in both bright filed and epi-fluorescence it's got to be objectives or
> somewhere above.
Be careful how you interpret this. I'm sure that Russell knows exactly
what he means, but unless you already know what he is saying it is
difficult to understand. Here is my expanded explanation.
1) If the artifacts are in the same position in each image frame as you
rotate the camera, then it is on the camera, or maybe on its mount.
2) If the artifacts rotate on the image as you rotate the camera then
they are somewhere earlier in the optical setup, probably the tube
lens, or a mirror in the camera body.
If they are nearly in focus (it sounds as though they are) then I
suspect they are on the front window of the CCD. These are not trivial
to clean!
Ian