Posted by
Bowman, Doug on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Not-a-confocal-question-tp592323p592334.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalThe dust could also be on the inside front service of the CCD window,
meaning that you won't be able to clean it. It would need to go back to
Hamamatsu.
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Ian Dobbie
Sent: Wednesday, March 12, 2008 2:10 PM
To:
[hidden email]
Subject: Re: Not a confocal question
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalRussell Spear <
[hidden email]> writes:
> Can you rotate the camera? If the "dirt" moves it with the camera or
> the mount. I it stays in same place it's somewhere in the microscope
> in both bright filed and epi-fluorescence it's got to be objectives or
> somewhere above.
Be careful how you interpret this. I'm sure that Russell knows exactly
what he means, but unless you already know what he is saying it is
difficult to understand. Here is my expanded explanation.
1) If the artifacts are in the same position in each image frame as you
rotate the camera, then it is on the camera, or maybe on its mount.
2) If the artifacts rotate on the image as you rotate the camera then
they are somewhere earlier in the optical setup, probably the tube
lens, or a mirror in the camera body.
If they are nearly in focus (it sounds as though they are) then I
suspect they are on the front window of the CCD. These are not trivial
to clean!
Ian
This e-mail, including any attachments, is a confidential business communication, and may contain information that is confidential, proprietary and/or privileged. This e-mail is intended only for the individual(s) to whom it is addressed, and may not be saved, copied, printed, disclosed or used by anyone else. If you are not the(an) intended recipient, please immediately delete this e-mail from your computer system and notify the sender. Thank you.