Narrow down the location of the artefacts. Obtain a live image and rotate your Orca at the C-Mount (where it attaches to the microscope). If the artefacts do not move with the image then there is something on the Orca’s sensor. If the artefacts move with the movement of your sample then the artefacts are somewhere in the microscope.
Michael Weis
Electron Microscopy & Digital Imaging
Pacific Agri-Food Research Centre
Agriculture and Agri-Food Canada
4200 Highway 97
Summerland, BC V0H 1Z0
Telephone: 250-494-6410
Facsimile: 250-494-0755
P Before printing think about the environment. / Avant d'imprimer, il faut penser à l'environnement.
-----Original Message-----
From: Confocal Microscopy List
[mailto:[hidden email]] On
Behalf Of Matthew Pearson
Sent: Wednesday, March 12, 2008
9:29 AM
To: [hidden email]
Subject: Not a confocal question
This is not specifically a confocal question but a microscopy question none the
less. We have a Zeiss axiovert 100M using a Hamamatsu Orca camera.
There seems to be an image artifact somewhere in the optical train. When
I have the live camera feed there are lots of small circles with smaller rings
inside. Could this be dust that has collected somewhere? I have tried
cleaning every surface I can get access to but it is still there! I get it
whether doing bright field or fluorescence and it is not dirty objective
lenses. It is not filters within the condenser either as the same
particles appear in the image irrespective of the condenser position. I
have removed the camera and cleaned it, still no look. So I'm at a loose
end really as to what it can be. I can only think it is something within
the microscope body beneath the objective housing and I don't really want to
dismantle the whole thing! Has anyone had similar problems in the past?
Thanks,
Matt Pearson.
-- Imaging TechnicianCell Biology DivisionInstitute of OphthalmologyUniversity College LondonEC1V 9EL020 7608 6857[hidden email] | Free forum by Nabble | Edit this page |