http://confocal-microscopy-list.275.s1.nabble.com/Not-a-confocal-question-tp592323p592336.html
this might be dust on either the camera or the tube lens. What happens
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> This is not specifically a confocal question but a microscopy question
> none the less. We have a Zeiss axiovert 100M using a Hamamatsu Orca
> camera. There seems to be an image artifact somewhere in the optical
> train. When I have the live camera feed there are lots of small circles
> with smaller rings inside. Could this be dust that has collected
> somewhere? I have tried cleaning every surface I can get access to but
> it is still there! I get it whether doing bright field or fluorescence
> and it is not dirty objective lenses. It is not filters within the
> condenser either as the same particles appear in the image irrespective
> of the condenser position. I have removed the camera and cleaned it,
> still no look. So I'm at a loose end really as to what it can be. I
> can only think it is something within the microscope body beneath the
> objective housing and I don't really want to dismantle the whole thing!
> Has anyone had similar problems in the past?
>
> Thanks,
>
> Matt Pearson.
>
>
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> Imaging Technician
> Cell Biology Division
> Institute of Ophthalmology
> University College London
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