Hi John, I have always had a high opinion
of LUDL products. Can you give an idea of how much this A1 LUDL set up cost
you? Is it more cost effective to get the motorized components from Zeiss or
LUDL?
Is your camera the Photometrics QuantEM?
How are you liking the EM camera (other than the problems with Axiovision
driver compatability)?
Cheers,
Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of
626-359-8111 x62872
From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Jon Ekman
Sent: Wednesday, February 27, 2008
1:40 PM
To: [hidden email]
Subject: Re: Thoughts on
Axioimager line of Microscopes/ Imaging system
I recently built an A1 using Ludl XY stage
and Z motor, all controlled through Ludl MAC 5000 controler. We use the system
for stereology. If you don’t get the upgrade, the A1 is still a very
flexible stand. Because we cover a lot of disciplines here, our A1 was built to
be capable of tiling, z-stacking, time series, transmitted light imaging,
polarized light imaging, fluorescence imaging (Ludle 10 port filter wheels) and
reflected light imaging. If you go with the motorized versions and add the
Axiovision software for control along with Zeiss cameras I think you will be
quite happy.
We have an inverted Axiovert 200M that can
do limited live cell work; it also does everything the A1 does, except it only has
five fluorescent channels (no filter wheels) and uses Axiovision software. The
key to success with the Axiovision software is to buy the Zeiss cameras also.
We have a Roper EMCCD and a Zeiss Axiocam and there are features like shading
correction that are locked out of the multidimensional acquisition setup in for
the Roper camera making it harder to work with than the Zeiss camera. You can
still do shading correction it just takes more steps.
Both A1 and the Axiovert 200M are used
quite heavily and require little maintenance. When a part does break it can be
exchanged at a significantly reduced cost through a parts exchange program.
Cheers,
Jonathan M. Ekman
Imaging Technology Group
Beckman Institute for
Advanced Science and
Tel: 217-244-6292
Fax: 217-244-6219
From:
Sent: Wednesday, February 27, 2008
12:08 PM
To: [hidden email]
Subject: Thoughts on Axioimager
line of Microscopes/ Imaging system
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello All,
I would appreciate hearing thoughts on Zeiss' line of Axioimager
imaging systems. I've just found out that an A1 (most basic, for
documentation) is going into our tiny (2 scope) facility. However, I am
thinking of asking for an upgrade to the M1, motorized and apparently suitable
for live cell imaging, 3D deconvolution microscopy, FRET even. Is anyone out
there using their Axiovision's for live cell imaging, FRET, 3D acquisition and
deconvolution?
Any and all thoughts are greatly appreciated.
Cheers
Farid
--
Farid Jalali MSc
Senior Research Technician/ Lab Manager
Dr. Robert Bristow Lab
Applied Molecular Oncology
416-946-4501 X4351 (
416-581-7754 STTARR at
416-581-7791 STTARR Microscopy Suite
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